玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

簡要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2068

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
大肉大捧一进一出| 人人妻人人澡人人爽欧美二区| 偷窥23个美女撒尿视频| 国产av无码成人黄网站免费| 国产精品久久久久成人免费| 处破学生毛都没长齐在线播放| 老太熟妇性BBWBBWBBW| 欧美精品黑人粗大视频| 人狗ZOOM欢迎你| 欧美性生交XXXXX久久久| 女的被弄到高潮娇喘喷水视频| 午夜少妇在线观看视频| 色戒电影未删减完整版免费观看| 初小VIDEOS第一次摘花| 暴虐SM调教A片| 宝贝的小嫩嫩好紧好爽H| 人妻斩り56歳无码| 国产放荡对白视频在线观看| 久久国产精品波多野结衣AV| 中文字幕在线观看| 色婷婷久久综合丁香五月狠狠野花 | 久久99国产综合精品| 亚洲一区二区嗯好爽快点| porno日本| 日本VA欧美VA欧美VA精品| 日日躁夜夜躁夜夜揉人人视频| 久久天天躁狠狠躁夜夜AV| 久久久久久久久| 国产一起色一起爱| 午夜亚洲AV永久无码精品| 粗大的内捧猛烈进出少妇| 厨房的春潮A片| 欧美性猛交XXXX富婆| 日本韩国男男作爱GAYWWW| 成人片黄网站A毛片免费| 精品久久久久中文字幕日本| 国产精品日日做人人爱| 六十路の高齢熟女が中文在线播放| 国产中文精品无码欧美综合小说| 国产精品无码一区二区| 成人片黄网站色大片免费|