玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1720F9 小鼠畸胎瘤細(xì)胞

F9 小鼠畸胎瘤細(xì)胞

簡(jiǎn)要描述:CRL-1720 F9 小鼠畸胎瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1720
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:1825

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1720 F9 小鼠畸胎瘤細(xì)胞


ATCC® Number:  CRL-1720™

Designations:  F9

Depositors:   S Strickland

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: epithelial

CRL-1720 F9 小鼠畸胎瘤細(xì)胞

Source: Organ: testis

Strain: 129

Disease: embryonal carcinoma; testicular teratoma

Cellular Products: plasminogen activator; laminin; type IV collagen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Age:  embryo

Comments: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP).

Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.

cAMP is active only on cells that have been treated with retinoic acid.

The cells maintain three copies of the beta 1 integrin gene.

Tested and found negative for ectromelia virus (mousepox).

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new coated culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719

1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238

23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677

26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215

32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649



CRL-1720


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲国产精品一区二区久久| 人久人久人久污污污精品国产| 中文字幕爆乳JULIA女教师| 国产韩国精品一区二区三区| 精品国产污污免费网站入口| 男男调教羞耻H扒开鞕臀海棠| 久久综合九色综合欧美狠狠| 饥渴少妇A片AAA毛片小说| 国产边打电话边做对白在线| 被吊起来用各种道具玩弄失禁| 午夜精品久久久久久中宇| 久久精品道一区二区三区| 好男人视频在线观看免费完整版| 女人和拘做受全过程免费| 特黄特色大片免费播放器图片 | 亚洲精品中文字幕| 重囗味SM在线观看无码| 国产对白嫖老妇搡老太| 亚洲精品无码久久久久SM| 国产精品久久久久久无码av片| 无码国产精品一区二区免费虚拟VR | 被主人惩罚在惩戒室SM| 军人各种做高H尿在里面| 一本色道久久爱久久综合网| 又色又爽又黄又粗暴的小说| 欧美ZC0O人与善交另类A片 | 被主人野外调教暴露羞辱历程| 2023国产麻豆剧传媒鱿鱼游戏| 精品无码AV一区二区三区不卡| 国产AV无码亚洲AV无码| 亚洲国产另类久久久精品黑人| 国产精品久久久久无码av色戒| 高洁在公车被灌满JING液| 97精品人妻系列无码人妻| 国产睡熟迷奷系列精品| 高雅人妻被迫沦为玩物| 中国极品艳妇AV少妇HD| 2022最新韩国理伦片在线观看| 国产后入清纯学生妹| 他一边曰一边吃我奶小说免看| 欧美大肥婆大肥bbbbb|