玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1503STO 小鼠胚成纖維細(xì)胞系

STO 小鼠胚成纖維細(xì)胞系

簡要描述:CRL-1503 STO 小鼠胚成纖維細(xì)胞系
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1503
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2140

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系 的詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系

ATCC® Number:CRL-1503™  Price:$289.00
Designations:STO

Depositors:G Martin

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Organ: embryo
Strain: SIM
Cell Type: fibroblast


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1503

Isolation:Toronto Ontario, Canada

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Age:embryo

Comments:The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

irradiated to be used as feeder cells:ATCC 56-X



References:1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

21874: . Teratomas and differentiation. New York: Academic Press; 1975.

22387: . . Cell 6: 467-474, 1975.

22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品国产亚洲AV麻豆图片| 日韩精品一区二区三区四区蜜桃| 乱人伦XXXX国语对白| japanese日本丰满少妇| 无人区乱码一区二区三区| 欧美人与性动交Α欧美精品| 粉嫩虎白扒开小泬| 亚洲AV无码一区二区一二区| 亚洲中文字幕久久精品无码喷水 | 夜夜性日日交XXX性HD| 小嫩模无套内谢第一次| 自W喷泉网站| 久久狠狠高潮亚洲精品| 两腿间花蒂被吸得肿了视频| 成全视频在线观看免费| 将军边走边挺进她的小泬| 极品美女扒开粉嫩小泬AV视频| 绝味儿媳妇TXT| 麻豆人妻无码性色AV专区| 日本髙清无码免费视频| 被亲妺妺夹得我好爽| 人人妻人人玩人人澡人人爽| 精品国产一二三产品区别在哪| 欧美激情第1页| 午夜亚洲国产理论片2020| 夜夜躁狠狠躁日日躁AAB| 亚洲国产成人精品无码区在线观看 | 亚洲AV无码偷拍在线观看| 女人与公拘交酡全过程| 天堂√最新版中文在线| 久久精品人人做人人爽电影蜜月| 国产伦精品一区二区三区视频| 入禽太深免费完整版电影| 五月槐花香电视剧全集免费| 中文无码成人精品久久久久| WWW色情成人免费视频软件| 色欧美片视频在线观看| 国产乱人伦偷精品视频免下载| 日本丰满大乳人妻无码| 欧美成人精品一区二区免费| gogo大胆欧美人体艺杧图片|