玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-105SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

簡(jiǎn)要描述:CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-105
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1746

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞的介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

ATCC® Number: CCL-105™ Price: $323.00

Designations: SW-13

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: adrenal gland

Tissue: cortex

Tumor Stage: grade IV

Disease: primary small cell carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Isolation date: August, 1971

Virus Susceptibility: Human poliovirus 1

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9

D16S539: 12

D5S818: 12

D7S820: 8,10

THO1: 7,8

TPOX: 8

vWA: 17,19

Cytogenetic Analysis: modal number = 63; range = 45 to 65.

Approximay 10% of the cells examined possessed a pair of dicentric chromosomes.

Isoenzymes: G6PD, B

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: Electron microscopic studies show many bulb gap junctions (BGJ).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22140: . . In Vitro 8: 443, 1973.

22526: Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537

26278: Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805

26279: Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896

CCL-105


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
60岁大白屁股熟妇真爽| 天堂AV无码AV一区二区三区| 富婆如狼似虎找黑人老外| 99久久久无码国产精品秋霞网| 可以玩的18款禁用游戏手游| 黑人60厘米全进去了| 一进去一爽又粗又大| 在线 | 一区二区三区| 无码粉嫩小泬无套在线观看A片| 强行剥开两边虐花蒂玩弄| 成免费CRM在线看| 又黄又爽又猛1000部A片| 亚洲av鲁丝一区二区三区| 蜜桃传媒一区二区亚洲AV婷婷| 色妞ww精品视频7777| 亚洲AV无码一区二区乱子伦| 人人妻人人玩人人澡人人爽| 日本潮水RAPPER超多| A级毛片无码免费真人久久| 香港60部三级未删版电影| 国产AV色情成人片在线播APP| 久久久久久精品免费看SSS| 成视频年人黄网站免费视频| 又小又紧女MAGNET| 极品人妻洗澡后被朋友玩| 凹凸精品视频视频学生AV| 国产精品久久久久久无码av片| 久久精品国产99国产精2018| 40厘米黑人与中国女人| 厨房里强摁做开腿呻吟| 亚洲色少妇熟女11p| 亚洲精品一区二区| 国产奶头好大揉着好爽视频| 日韩国产精品无码一区二区三区| 沧元图动漫全集免费观看高清| 一本加勒比HEZYO东京热高清 | 国产好大对白露脸高潮| 久久日本片精品AAAAA国产| 色欲久久99精品久久久久久av| 国产精品久久久久久久9999| 国产永久免费CRM系统有哪些|