玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2741HBE135-E6E7人支氣管上皮細(xì)胞

HBE135-E6E7人支氣管上皮細(xì)胞

簡要描述:HBE135-E6E7人支氣管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2741
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:3089

產(chǎn)品分類

Product Category

詳細(xì)介紹

HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.



細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells     under an inverted microscope until       cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping, do not agitate the cells     by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37°C.


Subc*tion Ratio: 1:3 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.

培養(yǎng)條件:Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat.  17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产美女视频国产视视频| 东北熟妇啪啪流白浆| 色8久久人人97超碰香蕉987| 粗大挺进尤物人妻中文字幕 | 中文日产乱幕九区无线码| 人人妻人人澡人人爽精品欧美| 欧洲美熟女乱又伦AV影片| 少妇放荡的呻吟干柴烈火| 特级毛片A级毛片免费观看网站| 人妻巨大乳一二三区| 亚洲精品乱码久久久久久| 国产suv精品一区二区| 亚洲精品久久国产高清| 扒开她粉嫩的小缝尿进去H漫画| 与子敌伦刺激对白亂輪亂性| 14又嫩又紧水又多| av无码一区二区三区| 风韵丰满熟妇啪啪区老熟熟女| 老师含紧一点H边做边走| 四虎成人精品在永久免费| 国产麻豆精品一区二区三区V视界| 亚洲中文字幕无码久久综合网| 色婷婷五月综合中文字幕| 九色丨PORNY丨自拍 ICU| 风韵丰满熟妇啪啪区老熟熟女| 1吨柴油等于多少升| 中文精品无码中文字幕无码专区| 嗯啊小SAO货奶头好硬视频| 乱人伦精品视频在线观看| 亚洲狠狠婷婷综合久久久久图片 | 亚洲一区二区三区| 97人人添人澡人人爽超碰| 亚洲成AV人综合在线观看| 97国产av传媒视频在线观看| 国产精品色情国产三级金瓶双艳| 同性两个17男互摸互吃的小说| 精品香蕉一区二区三区| 国产香蕉尹人视频在线| 中文字幕人妻无码乱精品| 国产真实伦对白全集| 国产精品国产精品国产专区不卡|