玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2553Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2553
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:2023

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2553™    Price:$417.00
Designations:Panc 02.03Depositors:EM JaffeeBiosafety Level:1edium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Shipped:frozenMMorphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655]DNA Profile (STR):Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 9,12 vWA: 17Age:70 yearsGender:femaleEthnicity:WhiteComments:Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:28.2 hrs [50655]Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
三A级做爰片免费观看玉蒲团 | porno日本| 免费国产又色又爽又黄的网站 | 久久人妻无码一区二区| 精品爆乳一区二区三区无码AV| 最近最新的日本字幕MV| 免费高清视频| 亚洲精品乱码久久久久久蜜桃| 久久人妻无码一区二区| 英语老师的小兔子好大好软水| 我把我的肥岳日出水来73| 色老太BBWBBWBBW高潮| 久久成人无码国产免费播放| 含羞草国产亚洲精品岁国产精品| 欧美人与善交大片免费看| 自拍偷自拍亚洲精品播放| 少妇BBW搡BBBB搡| 蜜臀久久AV无码牛牛影视| АⅤ天堂 中文在线| 999久久久国产精品| 小少爷撅着屁股挨C双龙若| 午夜精品久久久久久久无码| 亚洲色偷偷色噜噜狠狠99网| 亚洲国产精品无码久久A片小说| 99国产精品久久久久久久成人热| 人人澡人人澡人人看添AV| 99久久久免费精品国产| 久久精品熟女亚洲AV麻豆| 中文字幕乱码亚洲精品一区| 国产精品久久久久久AV| 香港三日本三级少妇三级2021| 白丝JK校花娇喘求饶白浆露出| 免费网站安全软件大全| 变态另类玩孕妇临盆| 久久艳务乳肉豪妇荡乳A片| 我的变态室友(H)三攻一受 | 小粉嫩精品A片在线视看| 色婷婷久久综合丁香五月狠狠野花 | 啦啦啦中文免费观看在线| 国产无遮挡a片又黄又爽| 无码欧亚熟妇人妻AV在线|