玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2553Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2553
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:2023

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2553™    Price:$417.00
Designations:Panc 02.03Depositors:EM JaffeeBiosafety Level:1edium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Shipped:frozenMMorphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655]DNA Profile (STR):Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 9,12 vWA: 17Age:70 yearsGender:femaleEthnicity:WhiteComments:Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:28.2 hrs [50655]Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品无码久久综合网| 在线视频免费观看| 成人禁片免费播放35分钟| 99久久人妻无码精品系列| 97人妻天天爽夜夜爽二区| 无码人妻丰满熟妇区五十路| 欧美乱人伦人妻中文字幕| 要灬要灬再深点受不了好舒服| 粗大黑人巨精大战欧美成人免费看| 国产男女爽爽爽免费视频| 女人被添全过程A一片| 精品国产A∨无码一区二区三区| 供人泄欲玩弄的妓子H| 日本添下边无码视频全过程| 国产精品视频一区二区| 久久久久99精品成人片| 久久久无码精品人妻一区| 被各种陌生人NP调教灌尿| 少妇人妻偷人精品一区二区| 寡妇玩XXXXXX猛男吃奶| 国产色欲色欲色欲.WWW| 与亲女洗澡时伦了视频| 偷窥23个美女撒尿视频| 办公室欧美大尺寸suv| 少妇SPA按摩按出水了| 少妇性活bbbbbbbbb四川| 色婷婷AV一区二区三区仙踪林| 久视频精品免费观看99| 丰满大爆乳波霸奶| А中文在线天堂| 精品国产AV一区二区三区| 亚洲AV无码乱码在线观看,不卡| 两个人免费完整版在线观看视频| 亚洲色无码A片一区二区| 国产亚洲精品久久久久久| 久久97久久97精品免视看秋霞| jizz性欧美2| 黄海川邱舒涵全文免费阅读| 国产成人艳妇AA视频在线| 特黄A级毛片| 欧美性猛交xxxx|