玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-1J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

簡要描述:J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:HTB-1
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1529

產(chǎn)品分類

Product Category

詳細(xì)介紹

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

ATCC® Number:HTB-1™ Price:
Designations:J82Biosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial Source:Organ: urinary bladder Disease: transitional cell carcinomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining thAntigen Expression:HLA A2, Aw32, B5, B12, Cw5; Bloode permits. Please click here for information regarding the specific requirements for shipment to your location.Tumorigenic:YesType ADNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 9.3 TPOX: 11,12 vWA: 17,18Cytogenetic Analysis:The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2Age:58 yearsGender:male                       J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞Ethnicity:Caucasian, SwedishComments:Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed. Contains the ras (H-ras) oncogene.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.22325: O'Toole C, et al. Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma. Br. J. Cancer 38: 64-76, 1978. PubMed: 68751924381: Fogh J. C*tion, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 57104725065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 901248432266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男男潮喷翻白眼漏尿高H漫画| 国产精品三级在线观看无码 | 中国熟妇色XXXX欧美老妇多毛 | 被几个领导玩弄一晚上| 永久黄网站色视频免费直播| 波多久久亚洲AV精品无码| 无码精品a∨在线观看十八禁| 最近中文字幕免费MV视频7| 久久99国产精一区二区三区| 无码日韩人妻精品久久蜜桃| 一本色道久久99一综合| 门卫老李干了校花高小柔| 精品无码一区二区三区| AV人摸人人人澡人人超碰妓女 | 中文字幕精品无码亚洲字幕蜜芽| 狂野欧美性猛交XXXX| 一本久久精品一区二区| 香蕉欧美成人精品av在线 | 亚洲AV中文无码字幕色三| 中文乱码人妻系列一区二区| 免费不卡在线观看AV| 黑人巨大JEEP日本人| 亚洲乱妇亚洲乱妇XINGLU| AAA级精品无码久久久国产片| 久久精品国产| 第一次破了女处小红| 国产精品videossex久久发布| 欧美ZC0O人与善交另类A片| 女人爽到高潮30分钟一次正常吗| 日韩精品人妻系列无码专区免费 | 超碰97久久国产精品牛牛| 与子敌伦刺激对白亂輪亂性| 亚洲AV无码国产精品久久不卡| 国产伦精品一区二区三区免费| 少妇人妻精品一区二区三区| 护士扒下内裤让我爽一夜| 无遮挡A级毛片免费看| 小SAO货CAO得你舒服吗男男| 亚洲av无码99久久久国精| 亚洲午夜AV久久久精品影院色戒 | 再深点灬舒服灬太大了添A片V |