玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1739AGS 人胃腺癌細(xì)胞

AGS 人胃腺癌細(xì)胞

簡(jiǎn)要描述:CRL-1739 AGS 人胃腺癌細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國(guó)典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號(hào):CRL-1739
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2260

產(chǎn)品分類

Product Category

詳細(xì)介紹

RL-1739 AGS 人胃腺癌細(xì)胞 的詳細(xì)介紹

AGS 人胃腺癌細(xì)胞

ATCC® Number:  CRL-1739™

Designations:  AGS

Depositors:   SC Barranco

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: stomach

Disease: gastric adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: 1979

Applications: transfection host (Nucleofection technology from Lonza)

Tumorigenic: Yes

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Age:  54 years

Gender:  female


Ethnicity:  Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22748: Barranco





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
好大好湿好硬顶到了好爽视频| 国内老熟妇对白XXXXHD| 玩弄丰满少妇高潮A片推油小说| 高中女学生破苞视频免费| 久久精品国产亚洲AV久| 精品国产18久久久久久| 少妇无力反抗慢慢张开双腿| 一本一道久久A久久精品综合 | YIN荡的老师系列第6部分| 中文在线无码高潮潮喷| 国产波霸爆乳一区二区| 久久久久国产精品| 精品白嫩BBWBBWBBW| 99精品欧美一区二区三区| 久久久久人妻一区精品色欧美 | 歪歪爽蜜臀AV久久精品人人槡| 香港三级日本三级妇三级| 八戒八戒在线观看免费完整版| 精品久久久久久久免费人妻| 波多野结衣AV| 熟妇人妻中文字幕| 中文字幕人妻紧无码专区| 亚洲AV乱码久久精品蜜桃| 女人被添全过程A片试看V| 亚洲国产精华液网站W| 男警察受呻吟双腿大开H漫画| 精品无码AV一区二区三区不卡| 日韩电影免费在线观看网址| 中国女人内谢69XXXXXA片| 免费一对一真人视频APP| 欧美成人一区二免费视频| 国产400部AV国片免费| 最近高清无吗免费看| ass鲜嫩鲜嫩pics日本| 无码国产69精品久久久久孕妇| 小12箩利洗澡无码视频网站| 毛片免费视频| 粉粉嫩嫩是AABB| 色哟哟免费精品网站入口| 不许穿内裤随时挨C调教H| 极品白嫩的小少妇|